Glutathione depletion and dual model oxygen balance disruption for photodynamic therapy enhancement ScienceDirect Could Tylenol be making your child's health worse? Acetaminophen is widely used for pain and fever relief, but research suggests it depletes glutathione, a powerful antioxidant essential for immune function, Identification of cytotoxic, glutathione reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.