Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter Multiscale modelling approach combining a kinetic model of glutathione metabolism with PBPK models of paracetamol and the potential glutathione depletion biomarkers ophthalmic acid and 5 oxoproline in humans and rats Integrative Biology (RSC A study published in Gastroenterology describes a new mitochondrial mediator in the hepatotoxicity induced by paracetamol. Institute of Biomedical Research of Barcelona Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.