Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Liver specific Bcl3 Knockout Alleviates Acetaminophen induced Liver Injury by Activating Nrf2 Pathway in Male Mice Cellular and Molecular Gastroenterology and Hepatology