Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione, often dubbed the master antioxidant plays a crucial role in detoxification, immune function, and protecting our cells from oxidative stress. Here are some signs that your levels might be low: Persistent What is glutathione? Know as the most important antioxidant in the body, glutathione is an essential antioxidant manufactured in all cells, but primarily in the liver. It is essential because it Glutathione depletion induces ferroptosis, autophagy, and premature cell senescence in retinal pigment epithelial cells Cell Death & Disease