Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity Researchers are studying a possible link between acetaminophen (Tylenol) use during pregnancy and higher autism or ADHD risk. It may lower glutathione, an important antioxidant for brain development. The Use and Potential Benefits of N Acetylcysteine in Non Acetaminophen Acute Liver Failure: An Etiology Based Review