Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. PDF) Erratum to: The effects of acute acetaminophen toxicity on hepatic mRNA expression of SOD, CAT, GSH Px, and levels of peroxynitrite, nitric oxide, reduced glutathione, and malondialdehyde in rabbit Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Tylenol an overview ScienceDirect Topics