The Use and Potential Benefits of N Acetylcysteine in Non Acetaminophen Acute Liver Failure: An Etiology Based Review Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Tylenol an overview ScienceDirect Topics Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.